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Statistically unbiased prediction enables accurate denoising of voltage imaging data
Here we report SUPPORT (statistically unbiased prediction utilizing spatiotemporal information in imaging data), a self-supervised …
Minho Eom, Seungjae Han, Pojeong Park, Gyuri Kim, Eun-Seo Cho, Jueun Sim, Kang-Han Lee, Seonghoon Kim, He Tian, Urs L. Böhm, Eric Lowet, Hua-an Tseng, Jieun Choi, Stephani Edwina Lucia, Seung Hyun Ryu, Márton Rózsa, Sunghoe Chang, Pilhan Kim, Xue Han, Kiryl D. Piatkevich, Myunghwan Choi, Cheol-Hee Kim, Adam E. Cohen, Jae-Byum Chang, Young-Gyu Yoon
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Statistically unbiased prediction enables accurate denoising of voltage imaging data
L-type Ca2+ channels mediate regulation of glutamate release by subthreshold potential changes
Subthreshold depolarization enhances neurotransmitter release evoked by action potentials and plays a key role in modulating synaptic …
Byoung Ju Lee, Unghwi Lee, Seung Hyun Ryu, Sukmin Han, Seung Yeon Lee, Jae Sung Lee, Anes Ju, Sunghoe Chang, Suk-Ho Lee, Sung Hyun Kim, Won-Kyung Ho
PDF DOI
L-type Ca2+ channels mediate regulation of glutamate release by subthreshold potential changes
DYRK3 phosphorylates SNAPIN to regulate axonal retrograde transport and neurotransmitter release
Among the five members of the dual-specificity tyrosine-phosphorylation-regulated kinase (DYRK) family, the cellular functions of DYRK3 …
Ye Hyung Lee, Bo Kyoung Suh, Unghwi Lee, Seung Hyun Ryu, Sung Ryong Shin, Sunghoe Chang, Sang Ki Park, Kwang Chul Chung
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DYRK3 phosphorylates SNAPIN to regulate axonal retrograde transport and neurotransmitter release
SCAMP5 mediates activity-dependent enhancement of NHE6 recruitment to synaptic vesicles during synaptic plasticity
Na+(K+)/H+ exchanger 6 (NHE6) on synaptic vesicle (SV) is critical for the presynaptic regulation of quantal size at the glutamatergic …
Unghwi Lee, Seung Hyun Ryu, Sunghoe Chang
PDF DOI
SCAMP5 mediates activity-dependent enhancement of NHE6 recruitment to synaptic vesicles during synaptic plasticity
SCAMP5 plays a critical role in axonal trafficking and synaptic localization of NHE6 to adjust quantal size at glutamatergic synapses
As a vesicular Na+(K+)/H+ exchanger, NHE6 promotes glutamate filling into synaptic vesicles (SVs). Thus, proper localization of NHE6 is critical for determining synaptic strength at gluta- matergic synapses, but the underlying mechanism remains un- known. We found that an SV-enriched protein, SCAMP5, is a key determinant for axonal trafficking and synaptic localization of NHE6. By perturbing their interaction, NHE6 fails to be localized at synaptic sites, resulting in hyperacidification of the SV lumen and a significant reduction in the quantal size of glutamate re- leased. Since both are autism candidate genes, our results sug- gest that impaired interaction between two proteins could relate to the synaptic dysfunction observed in autism.
Unghwi Lee, Chunghon Choi, Seung Hyun Ryu, Daehun Park, Sang-Eun Lee, Kitae Kim, Yujin Kim, Sunghoe Chang
PDF DOI
SCAMP5 plays a critical role in axonal trafficking and synaptic localization of NHE6 to adjust quantal size at glutamatergic synapses